Related Experiment Video
Updated: Apr 29, 2026

Soil Sampling and Isolation of Entomopathogenic Nematodes Steinernematidae, Heterorhabditidae
Published on: July 11, 2014
Demonstration of Coccidioides immitis and Coccidioides posadasii DNA in soil samples collected from Dinosaur National
Suzanne M Johnson1, Erin L Carlson2, Frederick S Fisher3
1Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, California smjohnson@ucdavis.edu.
Abstract:
Soil samples were collected in 2006 from Dinosaur National Monument (DNM), Utah, the site of an outbreak of coccidioidomycosis in 2001. DNA was isolated from two soil samples, and polymerase chain reaction (PCR) amplified Coccidioides DNA present in both samples. Ribosomal RNA genes and internal transcribed spacer (ITS) region PCR products were sequenced. Single-nucleotide polymorphisms indicated that the DNA from sample SS06RH was that of Coccidioides immitis, while the DNA from sample SS06UM was C. posadasii. This is the first report to directly demonstrate Coccidioides in soils from DNM and the first to report the presence of both C. immitis and C. posadasii in the same geographic location.
More Related Videos
11:09Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA
Published on: January 2, 2015
07:16Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples
Published on: January 6, 2022
Related Concept Videos
Other Unique Bacteria
In-situ Hybridization
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...