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An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
Published on: July 31, 2019
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Multiple omics uncovers host-gut microbial mutualism during prebiotic fructooligosaccharide supplementation
Tamotsu Kato1, Shinji Fukuda2, Akemi Fujiwara1
1Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan Graduate School of Nanobioscience, Yokohama City University, Kanagawa 230-0045, Japan.
Summary
Fructooligosaccharide (FOS) supplementation boosts beneficial gut bacteria and IgA production. This prebiotic
Area of Science:
- Microbiology
- Immunology
- Metabolomics
Background:
- Fructooligosaccharides (FOS) are prebiotics known for health benefits.
- The precise mechanisms by which FOS modulate the gut ecosystem are not fully understood.
- Interindividual variability complicates traditional gut microbiome and metabolite profiling.
Purpose of the Study:
- To elucidate the detailed mechanisms of FOS action in the human gut ecosystem.
- To investigate the relationship between FOS, microbial composition, metabolites, and immune responses.
- To establish a novel approach for analyzing the complex gut molecular network.
Main Methods:
- Employed a novel microbe-metabolite correlation approach.
- Integrated faecal immunoglobulin A (IgA) measurements.
- Utilized omics approaches for focused intraindividual analysis.
Main Results:
- FOS supplementation induced mucosal IgA production, correlating with specific bacterial presence.
- Metabolic dynamics of butyrate, L-phenylalanine, L-lysine, and tyramine positively correlated with these bacteria and IgA.
- p-cresol levels were negatively correlated with the identified bacteria and IgA production.
Conclusions:
- Intraindividual omics analysis is a powerful strategy for uncovering gut molecular networks.
- FOS modulates the gut ecosystem by influencing specific bacteria, metabolites, and IgA production.
- This study provides new insights into the human gut ecosystem and FOS mechanisms.
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