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Submicrogram quantities of unstained proteins are visualized on polyvinylidene difluoride membranes by
1Section on Neuroendocrinology, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, Maryland 20892.
Applied and Theoretical Electrophoresis : the Official Journal of the International Electrophoresis Society
|January 1, 1988
Abstract:
Unstained proteins on polyvinylidene difluoride membranes which have been dried and saturated with 20% methanol appear as clear areas when placed on a white light box. Sensitivity is comparable to that obtained with Coomassie Blue R250. This procedure greatly reduces the potential for unintended chemical modification during staining procedures, provides a nondestructive means of determining the major features of a protein pattern on a blot and makes it possible to initiate sequencing within minutes after blotting is complete.