Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

ATP and Macromolecule Synthesis01:28

ATP and Macromolecule Synthesis

5.1K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
5.1K
Radical Reactivity: Steric Effects01:10

Radical Reactivity: Steric Effects

1.6K
The presence of electron-donating, electron-withdrawing, or conjugating groups adjacent to a radical center, imparts electronic stabilization to the radicals. Examples of such electronically-stabilized radicals are triphenylmethyl, tetramethylpiperidine‐N‐oxide, and 2,2‐diphenyl‐1‐picrylhydrazyl. These radicals are remarkably stable and are known as persistent radicals. Some of the persistent radicals can even be isolated and purified.
Along with electronic...
1.6K
Complexation Equilibria: Factors Influencing Stability of Complexes01:09

Complexation Equilibria: Factors Influencing Stability of Complexes

995
In complexation reactions, metal cations are the electron pair acceptors, and the ligands are the electron pair donors. The stability of the metal complexes depends primarily on the complexing ability of the central metal ion and the nature of the ligands. Generally, the complexing ability of the metal ion depends on the size and charge of the ion. As the metal ion size increases, the stability of the metal complexes decreases, provided that the valency of the metal ion and the ligands remain...
995
Alkylation of β-Diester Enolates: Malonic Ester Synthesis01:14

Alkylation of β-Diester Enolates: Malonic Ester Synthesis

2.4K
Malonic ester synthesis is a method to obtain α substituted carboxylic acids from ꞵ-diesters such as diethyl malonate and alkyl halides.
2.4K
Regioselective Formation of Enolates01:33

Regioselective Formation of Enolates

2.6K
As depicted in the figure below, the unsymmetrical ketones can form two possible enolates: less substituted or more substituted enolates. Usually, the thermodynamic enolates are formed from the more substituted α-carbon atom, while the kinetic enolates are formed faster by deprotonation from the less substituted position. The thermodynamic enolates have lower energy, so they are more stable. But the energy required to form kinetic enolates is less.
2.6K
Cationic Chain-Growth Polymerization: Mechanism00:57

Cationic Chain-Growth Polymerization: Mechanism

2.1K
The cationic polymerization mechanism consists of three steps: initiation, propagation, and termination. In the initiation step of the polymerization process, the π bond of a monomer gets protonated by the Lewis acid catalyst, which is formed from boron trifluoride and water. The protonation of the π bond generates a carbocation stabilized by the electron‐donating group. In the propagation step, the π bond of the second monomer acts as a nucleophile and attacks the...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microfluidic affinity selection of active SARS-CoV-2 virus particles.

Science advances·2022
Same author

Identification of fetal aneuploidy with dual-probe fluorescence in situ hybridization analysis in circulating trophoblasts after enrichment using a high-sensitivity microfluidic platform.

Prenatal diagnosis·2021
Same author

ATG16 mediates the autophagic degradation of the 19S proteasomal subunits PSMD1 and PSMD2.

European journal of cell biology·2018
Same author

A polyphasic approach leads to seven new species of the cellulose-decomposing genus Sorangium, Sorangium ambruticinum sp. nov., Sorangium arenae sp. nov., Sorangium bulgaricum sp. nov., Sorangium dawidii sp. nov., Sorangium kenyense sp. nov., Sorangium orientale sp. nov. and Sorangium reichenbachii sp. nov.

International journal of systematic and evolutionary microbiology·2018
Same author

The vacuolar-type ATPase inhibitor archazolid increases tumor cell adhesion to endothelial cells by accumulating extracellular collagen.

PloS one·2018
Same author

The Translational Machinery of Human CD4<sup>+</sup> T Cells Is Poised for Activation and Controls the Switch from Quiescence to Metabolic Remodeling.

Cell metabolism·2018

Related Experiment Video

Updated: Apr 29, 2026

Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy
07:36

Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy

Published on: November 9, 2019

8.5K

Following nature's lead: generating compounds for stabilizing biomolecules.

Omo Clement1, Scott Whitney, Judy Muller-Cohn

  • 1Biomatrica, Inc. , San Diego, California.

Biopreservation and Biobanking
|May 23, 2014
PubMed
Summary

Researchers developed a novel method using biostability screens and computational chemistry to find small molecules for preserving biomolecules. This led to a successful DNA stabilization product and potential applications for RNA, proteins, and tissues.

More Related Videos

Rapid Generation of Amyloid from Native Proteins In vitro
05:48

Rapid Generation of Amyloid from Native Proteins In vitro

Published on: December 5, 2013

5.9K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

2.3K

Related Experiment Videos

Last Updated: Apr 29, 2026

Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy
07:36

Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy

Published on: November 9, 2019

8.5K
Rapid Generation of Amyloid from Native Proteins In vitro
05:48

Rapid Generation of Amyloid from Native Proteins In vitro

Published on: December 5, 2013

5.9K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

2.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Biomolecules like DNA, RNA, and proteins are susceptible to degradation in both dry and liquid states.
  • Preserving biomolecules is crucial for various applications, including diagnostics, therapeutics, and research.
  • Current methods for biomolecule stabilization have limitations in efficacy and scalability.

Purpose of the Study:

  • To develop a novel strategy for identifying small molecules that enhance biomolecule stability.
  • To investigate the efficacy of these small molecules in preserving DNA in the dry state.
  • To explore the potential of this approach for stabilizing other biomolecules such as RNA, proteins, and tissue samples.

Main Methods:

  • Utilized biostability screens combined with in silico docking and synthetic chemistry.
  • Analyzed libraries of small molecules for their protective capabilities on biological materials.
  • Assessed DNA-stabilizing compounds for their ability to form glasses at elevated temperatures.
  • Evaluated promising compounds for long-term DNA preservation efficacy.

Main Results:

  • Identified small molecules effective in stabilizing DNA in the dry state.
  • Developed a commercial product for DNA storage based on these findings.
  • Demonstrated the successful application of the screening approach for DNA preservation.
  • Showcased the potential for developing similar stabilizers for RNA, proteins, and tissue samples.

Conclusions:

  • The described approach offers a robust method for discovering small molecule stabilizers for biomolecules.
  • The developed DNA stabilization technology has significant commercial and experimental utility.
  • This strategy can be extended to create preservation solutions for a broader range of biological materials.