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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
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CD44 clustering is involved in monocyte differentiation.

Guoliang Zhang1, Huizhen Zhang2, Yiwen Liu1

  • 1Department of Molecular Biology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.

Acta Biochimica Et Biophysica Sinica
|May 23, 2014
PubMed
Summary

CD44, an adhesion molecule, plays a key role in monocyte differentiation into macrophages. Blocking CD44 inhibits differentiation markers and ERK1/2 phosphorylation, revealing its mechanism.

Keywords:
CD44activated stateclusteringmonocyte differentiationp-ERK1/2

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Area of Science:

  • Cell Biology
  • Immunology
  • Molecular Biology

Background:

  • Monocyte differentiation into macrophages is crucial in health and disease, but mechanisms remain unclear.
  • Adhesion molecules, like CD44, are implicated in cell differentiation.
  • CD44 exhibits distinct states in monocytes versus macrophages, suggesting a role in differentiation.

Purpose of the Study:

  • To investigate the role of CD44 in monocyte differentiation.
  • To elucidate the underlying mechanisms of CD44-mediated monocyte differentiation.

Main Methods:

  • Utilized THP-1 cells, a human monocytic leukemia cell line, as an in vitro model.
  • Induced differentiation using phorbol 12-myristate 13-acetate (PMA).
  • Blocked CD44 function to assess its impact on differentiation markers and signaling pathways.

Main Results:

  • PMA induction significantly increased CD44 expression, hyaluronic acid binding, and CD44 clustering.
  • Blocking CD44 inhibited PMA-induced CD44 clustering, high expression, and CD14 expression.
  • CD44 blockade also suppressed PMA-induced phosphorylation of ERK1/2 signaling pathway.

Conclusions:

  • CD44 is involved in the process of monocyte differentiation.
  • CD44 activation, through high expression and clustering, contributes to monocyte differentiation via ERK1/2 signaling.