Antibiotic resistance patterns of Enterococcus spp. isolated from Musca domestica and Chrysomya megacephala in ubon

Abstract

Insights

Houseflies (Musca domestica) and blowflies (Chrysomya megacephala) can carry and transfer antibiotic-resistant Enterococcus bacteria to humans. This study highlights their role as potential vectors of antimicrobial resistance.

Area of Science:

  • Medical Entomology
  • Microbiology
  • Public Health

Background:

  • Houseflies (Musca domestica) and blowflies (Chrysomya megacephala) are globally prevalent insects.
  • These flies are recognized as significant mechanical vectors of pathogens, transmitting diseases from unsanitary environments to food sources.

Purpose of the Study:

  • To investigate the antimicrobial susceptibility of Enterococcus species isolated from Musca domestica and Chrysomya megacephala.
  • To assess the potential of these flies to transfer multi-drug resistant Enterococcus to humans.

Main Methods:

  • Collected 700 adult flies (Musca domestica and Chrysomya megacephala) from various public locations.
  • Isolated and identified Enterococcus species, then performed antimicrobial susceptibility testing using disk diffusion and minimal inhibitory concentration (MIC) assays.

Main Results:

  • 120 Enterococcus isolates were obtained from 67 Musca domestica and 53 Chrysomya megacephala.
  • High susceptibility was observed for ampicillin (99.2%), chloramphenicol (74.2%), and tetracycline (75.0%).
  • Minimal inhibitory concentrations (MICs) varied for vancomycin, tetracycline, chloramphenicol, and ciprofloxacin.

Conclusions:

  • Musca domestica and Chrysomya megacephala possess the capability to harbor and potentially transmit Enterococcus species.
  • Nine distinct antimicrobial susceptibility patterns were identified among the isolated enterococci, indicating diverse resistance profiles.

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