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Updated: Apr 29, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Antibiotic resistance patterns of Enterococcus spp. isolated from Musca domestica and Chrysomya megacephala in ubon
Background:
The housefly Musca domestica and the blowfly Chrysomya megacephala are found worldwide and are medically significant as mechanical vectors of various pathogens from unsanitary locations to food, resulting in diseases in humans.
Objectives:
This study aimed to test the antimicrobial activity against Enterococcus spp. isolated from M. domestica and C. megacephala by standard disk diffusion and minimal inhibitory concentration (MIC), and to study the potential of M. domestica and C. megacephala to transfer multi-drug resistant enterococcus to humans.
Material And Method:
Seven hundred adult flies were collected from fresh-food markets, garbage piles, restaurants, school cafeterias, and rice paddy fields in Muang Ubon Ratchathani and Warinchamrap in Ubon Ratchathani Province. Antimicrobial susceptibility for Enterococcus spp. isolated from adult flies was performed by disk diffusion test and minimum inhibitory concentration (MIC) determination.
Results:
One hundred and twenty isolates of Enterococcus spp. were taken from 67 M. domestica and 53 C. megacephala. Standard disk diffusion showed the Enterococcus spp. isolates exhibited susceptibility to ampilcillin (99.2%), chloramphenicol (74.20%), tetracycline (75.0%), vancomycin (50.8%), and erythromycin (42.5%). The MICs of antimicrobial agents for all isolates were < or = 0.25-8 microg/mL for vancomycin, 1- > 16 microg/mL for tetracycline, 4- > 16 microg/mL for chloramphenicol, and 0.5-8 microg/mL for ciprofloxacin.
Conclusion:
The study demonstrated the potential of M. domestica and C. megacephala to carry Enterococcus spp. Nine antimicrobial susceptibility patterns were obtained among the 120 enterococci isolates.
Insights
Houseflies (Musca domestica) and blowflies (Chrysomya megacephala) can carry and transfer antibiotic-resistant Enterococcus bacteria to humans. This study highlights their role as potential vectors of antimicrobial resistance.
Area of Science:
- Medical Entomology
- Microbiology
- Public Health
Background:
- Houseflies (Musca domestica) and blowflies (Chrysomya megacephala) are globally prevalent insects.
- These flies are recognized as significant mechanical vectors of pathogens, transmitting diseases from unsanitary environments to food sources.
Purpose of the Study:
- To investigate the antimicrobial susceptibility of Enterococcus species isolated from Musca domestica and Chrysomya megacephala.
- To assess the potential of these flies to transfer multi-drug resistant Enterococcus to humans.
Main Methods:
- Collected 700 adult flies (Musca domestica and Chrysomya megacephala) from various public locations.
- Isolated and identified Enterococcus species, then performed antimicrobial susceptibility testing using disk diffusion and minimal inhibitory concentration (MIC) assays.
Main Results:
- 120 Enterococcus isolates were obtained from 67 Musca domestica and 53 Chrysomya megacephala.
- High susceptibility was observed for ampicillin (99.2%), chloramphenicol (74.2%), and tetracycline (75.0%).
- Minimal inhibitory concentrations (MICs) varied for vancomycin, tetracycline, chloramphenicol, and ciprofloxacin.
Conclusions:
- Musca domestica and Chrysomya megacephala possess the capability to harbor and potentially transmit Enterococcus species.
- Nine distinct antimicrobial susceptibility patterns were identified among the isolated enterococci, indicating diverse resistance profiles.
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