Related Experiment Videos
Methicillin-resistant Staphylococcus aureus with reduced susceptibility to vancomycin in Sanprasitthiprasong Hospital
Background:
Staphylococcus aureus is a species of bacteria that causes a number of diseases and more than 60% of it is presently resistant to methicillin. Vancomycin is the drug of choice for the eradication of methicillin-resistant S. aureus (MRSA).
Objective:
This study aimed to investigate the susceptibility of heterogeneous vancomycin intermediate S. aureus (hVISA) and vancomycin intermediate S. aureus (VISA) to vancomycin by standard disk diffusion, microbroth dilution, a one-point population assay, and a population analysis profile.
Material And Method:
Sixty-eight MRSA isolates from patients admitted to Sanprasitthiprasong Hospital between November 2010 and November 2011 were tested.
Results:
Standard disk diffusion showed that all the MRSA isolates were susceptible to vancomycin. Vancomycin MICs for all isolates were 1-2 microg/mL. Only two MRSA isolates (2.9%) were able to grow on brain heart infusion agar supplemented with vancomycin 4 microg/mL and were confirmed by a population analysis as hVISA.
Conclusion:
This study showed the effect of vancomycin on MRSA and the need for early detection and controlled planning.