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[Plasma microRNA profile in immune thrombocytopenia: screening and verification]
1Department of Hematology, Tianjin First Center Hospital, Tianjin 300192, China.
Objective:
To screen the plasma microRNA (miRNA) profile of immune thrombocytopenia (ITP) patients.
Methods:
Agilent 19.0 miRNA microarray was used to detect the expression profile of miRNA in plasma from 25 ITP patients and 20 healthy controls from June 2012 to September 2013. The software programs of TargetScan and miRanda were used for predicting target genes associated with differential miRNA. Then gene ontology (GO) and pathway analysis were performed to explore the genes and pathways involved in the pathogenesis of ITP. And differential miRNA was validated by real-time quantitative polymerase chain reaction (PCR).
Results:
A genome-wide miRNA array revealed 29 differential miRNAs in the plasma samples of ITP patients including 15 up-regulated and 14 down-regulated miRNA. A total of 608 potential genes were predicted by TargetScan and miRanda.GO result showed that there were 475 (78.12%), 491(80.76%) and 533 (87.66%) genes respectively involved in biological process, molecular function and cellular component.Enrichment test showed 9 GO terms had significant difference (P < 0.05). Pathway analysis showed that 157 pathways were associated with 608 genes.Enrichment test showed 25 pathways had significant difference (P < 0.05). As revealed by real-time PCR, the expressions of miRNA4778-5p and miRNA4800-5p became obviously up-regulated while those of miRNA4707-5p, miRNA4721, miRNA3620-3p and miRNA378i decreased (all P < 0.05). The results agreed with those of microarray.
Conclusions:
The plasma differential miRNA profiles are identified in ITP patients. And miRNA is involved in calcium signaling pathway and T cell receptor signaling pathway may be associated with ITP pathogenesis.
Insights
Researchers identified 29 differential microRNAs (miRNAs) in the plasma of immune thrombocytopenia (ITP) patients. These findings suggest specific miRNA profiles are linked to ITP and may involve calcium and T cell receptor signaling pathways.
Area of Science:
- Molecular Biology
- Genomics
- Immunology
Context:
- Immune thrombocytopenia (ITP) is an autoimmune disorder characterized by low platelet counts.
- Plasma microRNA (miRNA) profiles are increasingly recognized as potential biomarkers for various diseases.
- Understanding the molecular mechanisms underlying ITP is crucial for developing effective treatments.
Purpose:
- To screen and identify differential plasma microRNA (miRNA) expression profiles in patients with immune thrombocytopenia (ITP).
- To predict target genes and analyze their involvement in the pathogenesis of ITP using bioinformatics tools.
- To validate the expression levels of specific differentially expressed miRNAs in ITP patients.
Summary:
- A genome-wide miRNA microarray analysis revealed 29 differentially expressed miRNAs (15 up-regulated, 14 down-regulated) in the plasma of 25 ITP patients compared to 20 healthy controls.
- Bioinformatic analysis predicted 608 potential target genes, with Gene Ontology and pathway analyses indicating significant involvement in biological processes, molecular functions, cellular components, and 25 distinct pathways.
- Real-time quantitative polymerase chain reaction (PCR) validated the differential expression of several miRNAs, confirming the microarray findings and highlighting their potential role in ITP pathogenesis, particularly in calcium and T cell receptor signaling pathways.
Impact:
- Identifies novel plasma miRNA biomarkers for immune thrombocytopenia (ITP).
- Provides insights into the molecular pathways, including calcium and T cell receptor signaling, implicated in ITP pathogenesis.
- Establishes a foundation for further research into miRNA-based diagnostics and therapeutics for ITP.
