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Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Transgenic Organisms

Transgenic Organisms

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Updated: Apr 29, 2026

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

Design rules for efficient transgene expression in plants.

Mark A Jackson¹, Peter R Sternes, Stephen R Mudge

¹The University of Queensland, Brisbane, Qld, Australia.

Plant Biotechnology Journal

|May 24, 2014

Summary

This summary is machine-generated.

Researchers developed gene design rules to prevent transgene silencing in plants. Removing RNA instability sequences significantly improved stable gene expression, offering a simple method for plant biotechnology.

Keywords:

expression mRNA reporter sugarcane transgene transgenic

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Area of Science:

Plant Biotechnology
Molecular Biology
Genetics

Background:

Transgene silencing obstructs stable gene expression in plants, a common requirement in biotechnology.
Developing methods to ensure sustained transgene expression is crucial for advancing plant genetic engineering.

Purpose of the Study:

To establish and validate a set of gene design rules to overcome transgene silencing in plants.
To enhance the stability and expression levels of transgenes in sugarcane.

Main Methods:

Tested gene design rules including rare codon removal, RNA instability sequence elimination, and sRNA binding site blocking.
Analyzed stable transgene expression in multiple independent transgenic lines using northern blot analysis.
Applied Gene Designer 2.0 software for motif elimination and sequence randomization.

Main Results:

Removal of RNA instability sequences provided the greatest improvement in stable transgene expression.
Transcript degradation was significantly reduced, as evidenced by northern blot analysis.
Achieved 935- and 5-fold increased expression for luc and ims genes, respectively, with sustained expression over several years.

Conclusions:

The developed gene design rules effectively prevent transgene silencing in plants.
These rules offer a simple and broadly applicable method for achieving sustained transgene expression in various plant species.
The findings have significant implications for improving transgenic crop development and plant biotechnology.