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Updated: Apr 29, 2026

Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro
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Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro

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RECQL1 plays an important role in the development of tongue squamous cell carcinoma

Jiang Tao1, Suxiong Tao, Junli Han

  • 1Department of General Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|May 24, 2014
PubMed
Abstract

Insights

Silencing RECQL1 (RecQ-like helicase 1) inhibits tongue squamous cell carcinoma (SCC) growth and progression. This study demonstrates RECQL1

Area of Science:

  • Molecular biology
  • Oncology
  • Genetics

Background:

  • RECQL1, a RECQ helicase family member, is involved in DNA repair.
  • RECQL1 silencing causes mitotic catastrophe and inhibits tumor growth in models.
  • Therapeutic potential of RECQL1 in tongue squamous cell carcinoma (SCC) is unexplored.

Purpose of the Study:

  • To investigate the role of RECQL1 in tongue SCC development.
  • To evaluate the effects of RECQL1 silencing in tongue SCC cell lines.
  • To explore RECQL1 as a potential therapeutic target in tongue SCC.

Main Methods:

  • RNA interference (RNAi) was used to silence RECQL1 in human tongue SCC cell lines (SCC-9, SCC-15).
  • In vitro and in vivo assays were performed to assess the effects of RECQL1 silencing.
  • miR-203 expression and its regulatory role on RECQL1 were investigated.

Main Results:

  • RECQL1 mRNA and protein levels were downregulated upon siRNA-mediated silencing.
  • miR-203 was confirmed to downregulate RECQL1 post-transcriptionally.
  • RECQL1 knockdown or miR-203 overexpression inhibited SCC cell proliferation and induced apoptosis.
  • RECQL1 knockdown suppressed SCC-9 cell tumorigenicity in vivo.
  • RECQL1 knockdown and miR-203 overexpression reduced immunosuppressive factors in vitro.

Conclusions:

  • RECQL1 plays a significant role in regulating tongue SCC cell proliferation.
  • RECQL1 is a potential therapeutic target for tongue SCC treatment.
  • miR-203 acts as a tumor suppressor by downregulating RECQL1 in tongue SCC.

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