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Analysis of Epididymal Protein Synthesis and Secretion
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Lactoferrin expression and secretion in the stallion epididymis.

Christopher A Pearl1, Janet F Roser2

  • 1Department of Biological Sciences, Western Michigan University, 1903 West Michigan Avenue, Kalamazoo, MI 49008, USA; Department of Animal Science, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.

Reproductive Biology
|May 27, 2014
PubMed
Summary
This summary is machine-generated.

Stallion epididymal lactoferrin is produced by principal cells in mature animals and binds to sperm. Estradiol, not testosterone, regulates its secretion in the cauda epididymis, suggesting a role in sperm health.

Keywords:
EpididymisEstrogenLactoferrinSpermStallion

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Area of Science:

  • Reproductive biology
  • Animal science
  • Biochemistry

Background:

  • Lactoferrin is abundant in stallion epididymal secretions.
  • Its cellular origin and regulatory mechanisms in the epididymis are largely unknown.

Purpose of the Study:

  • To identify epididymal cells producing lactoferrin in stallions of different ages.
  • To determine if lactoferrin binds to stallion sperm.
  • To investigate the regulation of lactoferrin secretion by testosterone and estradiol.

Main Methods:

  • Immunohistochemistry was used to localize lactoferrin.
  • Sperm were examined for lactoferrin binding.
  • In vitro tissue culture and ELISA were employed to assess hormonal regulation of secretion.

Main Results:

  • Lactoferrin was localized in principal cells of the corpus and cauda epididymis in peri- and post-pubertal stallions, but not pre-pubertal ones.
  • Lactoferrin was detected on the mid-piece and tail of stallion sperm.
  • Estradiol-17β significantly increased lactoferrin secretion from cauda epididymis tissue, while testosterone had no effect.

Conclusions:

  • Lactoferrin expression in the stallion epididymis is age-dependent and localized to principal cells.
  • Lactoferrin binding to sperm suggests a role in sperm protection or regulation.
  • Estrogen plays a role in regulating lactoferrin secretion in the stallion cauda epididymis.