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A novel functional conducting polymer as an immobilization platform.

Emine Guler1, Hakan Can Soyleyici2, Dilek Odaci Demirkol1

  • 1Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova, Izmir, Turkey.

Materials Science & Engineering. C, Materials for Biological Applications
|May 27, 2014
PubMed
Summary

We developed novel conducting polymer biosensors using HKCN polymer for immobilizing pyranose oxidase (PyOx) and Gluconobacter oxydans. These HKCN/PyOx and HKCN/G. oxydans biosensors show sensitive glucose detection.

Keywords:
BiosensingConducting polymersG. oxydansPyranose oxidase

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Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Polymer Science

Background:

  • Conducting polymers offer versatile platforms for biosensor development.
  • Immobilization of enzymes and microbial cells is crucial for biosensor stability and performance.
  • The HKCN polymer provides free amino groups for efficient bioconjugation.

Purpose of the Study:

  • To fabricate novel conducting polymer-based enzymatic and microbial biosensors.
  • To utilize the HKCN polymer as an immobilization platform for pyranose oxidase (PyOx) and Gluconobacter oxydans.
  • To characterize the analytical performance of the developed glucose biosensors.

Main Methods:

  • Electropolymerization of HKCN on a graphite electrode.
  • Immobilization of PyOx and G. oxydans using glutaraldehyde cross-linking.
  • Analytical characterization using batch systems and flow injection analysis (FIA).

Main Results:

  • Developed HKCN/PyOx and HKCN/G. oxydans biosensors for glucose detection.
  • Observed concentration-dependent current changes in batch systems (0.05-1.0mM for HKCN/PyOx, 0.25-2.5mM for HKCN/G. oxydans).
  • Achieved linear ranges of 0.01-1.0mM (HKCN/PyOx) and 0.1-7.5mM (HKCN/G. oxydans) in FIA mode.

Conclusions:

  • The HKCN conducting polymer is effective for creating stable enzymatic and microbial biosensors.
  • The developed biosensors demonstrate sensitive and reliable glucose detection capabilities.
  • The HKCN polymer offers a promising platform for advanced biosensor applications.