Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

3.4K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deoxygenative Arylation of Allylic/Benzylic Esters through Cooperative Photoredox/Nickel Catalysis.

Organic letters·2026
Same author

The SIK3-N783Y mutation is associated with the human natural short sleep trait.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Time-Dependent Regulation of Sleep-Wakefulness and Electroencephalographic Characteristics by Spontaneous Running in Male Mice.

Journal of sleep research·2025
Same author

Identification of immune patterns in idiopathic pulmonary fibrosis patients driven by PLA2G7-positive macrophages using an integrated machine learning survival framework.

Scientific reports·2024
Same author

Diverse roles of pontine NPS-expressing neurons in sleep regulation.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Mutations in Metabotropic Glutamate Receptor 1 Contribute to Natural Short Sleep Trait.

Current biology : CB·2020

Related Experiment Video

Updated: Apr 29, 2026

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System
07:34

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System

Published on: December 16, 2022

3.0K

PER1 phosphorylation specifies feeding rhythm in mice.

Zhiwei Liu1, Moli Huang2, Xi Wu1

  • 1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing 210061, China.

Cell Reports
|May 27, 2014
PubMed
Summary

The PER1 gene mutation accelerates the body clock, causing advanced feeding behavior and obesity. This highlights PER1

More Related Videos

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
05:46

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Published on: January 24, 2013

21.2K
A Do-it-yourself System for Scheduled Feeding of Laboratory Rodents in Their Home Cage
04:49

A Do-it-yourself System for Scheduled Feeding of Laboratory Rodents in Their Home Cage

Published on: June 6, 2025

1.1K

Related Experiment Videos

Last Updated: Apr 29, 2026

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System
07:34

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System

Published on: December 16, 2022

3.0K
Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
05:46

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Published on: January 24, 2013

21.2K
A Do-it-yourself System for Scheduled Feeding of Laboratory Rodents in Their Home Cage
04:49

A Do-it-yourself System for Scheduled Feeding of Laboratory Rodents in Their Home Cage

Published on: June 6, 2025

1.1K

Area of Science:

  • Chronobiology
  • Metabolic regulation
  • Molecular genetics

Background:

  • Circadian rhythms are crucial for physiological and metabolic health.
  • Familial advanced sleep-phase syndrome (FASPS) is linked to mutations in the hPER2 gene.
  • The function of the paralogous PER1 phosphorylation site (S714) in circadian organization is unknown.

Purpose of the Study:

  • To investigate the role of the PER1 S714 phosphorylation site in circadian organization and metabolic regulation.
  • To compare the effects of PER1 and PER2 mutations on circadian behavior and metabolism.

Main Methods:

  • Generation and analysis of PER1 S714G mutant mice.
  • Assessment of molecular clock feedback loop kinetics.
  • Monitoring of feeding behavior and energy expenditure patterns.
  • Analysis of metabolic gene expression in liver and adipose tissue.
  • Evaluation of diet-induced obesity.

Main Results:

  • The PER1 S714G mutation accelerates the molecular circadian feedback loop.
  • PER1 S714G mice display advanced feeding behavior, with food intake peaking before energy expenditure.
  • Disrupted phase of metabolic regulators in liver and adipose tissue of PER1 S714G mice.
  • PER1 S714G mice rapidly develop obesity when fed a high-fat diet, unlike PER2 S662G mice.
  • PER1 and PER2 mutations affect distinct downstream pathways.

Conclusions:

  • PER1 plays a critical role in maintaining the alignment between the circadian clock and energy metabolism.
  • PER1 mutations can lead to metabolic disruption and obesity.
  • PER1 and PER2 regulate different aspects of circadian organization and metabolic control.