Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Potential role of tryptophan metabolites in the sex-based differences in doxorubicin-induced chronic cardiotoxicity in a rat model.

American journal of physiology. Heart and circulatory physiology·2026
Same author

Quantifying intracellular mechanosensitive response upon spatially defined mechano-chemical triggering.

eLife·2026
Same author

Prevalent gut phages encode modular adhesins mediating epithelial binding and endoplasmic reticulum trafficking.

Nature communications·2026
Same author

Tilorone suppresses TGF-β-driven fibroblast activation and restores BMP-Smad1/5/8 signaling: implications for laryngotracheal fibrosis.

Journal of translational medicine·2026
Same author

Role of Ca2+-activated KCa3.1 potassium channel in CAR T cell effector function.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Effects of Sulfate Metabolites of Chrysin, Quercetin, Luteolin, and Myricetin on the Albumin Binding of Warfarin (Site I) and Biliverdin (Heme Site): from Theoretical to Practical Considerations.

ACS omega·2026

Related Experiment Video

Updated: Apr 29, 2026

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
04:49

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Published on: September 2, 2008

11.6K

An in vitro system to study nuclear envelope breakdown.

Joseph Marino1, Lysie Champion1, Cornelia Wandke1

  • 1Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.

Methods in Cell Biology
|May 27, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed an in vitro system to study nuclear envelope breakdown (NEBD) during cell division. This new method allows detailed observation of NEBD mechanisms and identification of key molecular players involved in nuclear reorganization.

Keywords:
In vitro systemMicroscopyMitosisNuclear envelopeNuclear envelope breakdownNuclear membraneNuclear pore complexNucleoporin

More Related Videos

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
08:56

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

Published on: January 11, 2012

10.9K
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
08:29

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

Published on: May 14, 2018

9.5K

Related Experiment Videos

Last Updated: Apr 29, 2026

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
04:49

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Published on: September 2, 2008

11.6K
In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
08:56

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

Published on: January 11, 2012

10.9K
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
08:29

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

Published on: May 14, 2018

9.5K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Nuclear envelope breakdown (NEBD) is a critical event during vertebrate cell mitosis, involving the disassembly of the nuclear pore complexes, nuclear lamina, and nuclear membranes.
  • Understanding the molecular mechanisms governing NEBD is essential for deciphering cell division processes.
  • Existing methods may limit the detailed analysis of the dynamic changes occurring during NEBD.

Purpose of the Study:

  • To establish a novel in vitro experimental system that recapitulates nuclear envelope breakdown (NEBD).
  • To provide a powerful tool for observing NEBD dynamics and characterizing the molecular factors involved.
  • To facilitate the study of nuclear envelope reorganization during mitosis.

Main Methods:

  • Development of an in vitro assay using semi-permeabilized HeLa cells expressing fluorescently tagged nuclear envelope (NE) marker proteins.
  • Induction of NEBD by adding mitotic cell extract supplemented with fluorescently labeled dextran.
  • Time-lapse confocal microscopy to monitor NE marker protein dynamics and dextran influx, indicating loss of the NE permeability barrier.

Main Results:

  • The developed in vitro system successfully recapitulates the key events of nuclear envelope breakdown (NEBD).
  • The system allows for real-time monitoring of NE marker protein behavior during NEBD.
  • Loss of the nuclear envelope permeability barrier during NEBD can be effectively assessed using fluorescent dextran influx.

Conclusions:

  • This imaging-based in vitro system offers a robust platform for studying the intricate process of nuclear envelope breakdown (NEBD).
  • The system enables the identification and functional characterization of molecular components crucial for NE dynamics during mitosis.
  • It serves as a valuable tool for advancing our understanding of nuclear envelope reorganization in cell division.