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Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
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Inhibitory effect of nitrite on coagulation processes demonstrated by thrombelastography.
J W Park1, B Piknova1, K Nghiem2
1Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, United States.
Nitric Oxide : Biology and Chemistry
|May 27, 2014
Summary
Nitrite ions, under low oxygen, inhibit blood clotting by reducing platelet aggregation and clot strength. This suggests nitrite
Area of Science:
- Biochemistry
- Hematology
- Physiology
Background:
- Nitric oxide (NO) is produced via nitrate-nitrite reduction or from arginine by nitric oxide synthase (NOS).
- Nitrite ions, especially with erythrocytes under deoxygenation, inhibit platelet aggregation and activation.
- The effect of nitrite on overall blood clotting processes requires further investigation.
Purpose of the Study:
- To investigate the impact of nitrite on blood clotting dynamics using thrombelastography (TEG).
- To assess the influence of nitrite and an NO donor on platelet function and clot formation.
- To explore the role of hypoxic conditions in enhancing nitrite's effects on clotting.
Main Methods:
- Thrombelastography (TEG) was used to measure reaction time (R), alpha angle (α), and maximum amplitude (MA).
- Experiments were conducted on whole blood and platelet-rich plasma (PRP) from healthy volunteers.
- The effects of an NO donor (DEANONOate) and nitrite ions were assessed under varying oxygen conditions and hematocrit levels.
Main Results:
- Both DEANONOate and nitrite dose-dependently inhibited clotting parameters (R, α, MA) in whole blood and PRP.
- Nitrite's inhibitory effects were significantly enhanced under hypoxic conditions, becoming detectable at low concentrations (0.1 microM).
- Neither compound affected TEG parameters in plasma, indicating platelet and/or erythrocyte involvement.
Conclusions:
- Nitrite modulates overall blood clotting processes, primarily by affecting platelet function.
- Thrombelastography (TEG) is a suitable method for monitoring nitrite's impact on hemostasis.
- Endogenous nitrite levels could serve as potential biomarkers for predicting hemostatic potential.
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