Taxonomic database and cut-off value for processing mcrA gene 454 pyrosequencing data by MOTHUR
Related Experiment Video
Updated: Apr 29, 2026
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
85.9K
Sizhong Yang1, Susanne Liebner1, Mashal Alawi1
1GFZ German Research Centre for Geosciences, Section 4.5: Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.
Journal of Microbiological Methods
|May 27, 2014
Abstract:
The functional mcrA gene of methanogens can generate phylogeny as congruent as the 16S rRNA gene phylogeny. For the mcrA sequences amplified by mlas/mcrA-rev primers, we created a database for taxonomical classification and propose cut-off values for OTU clustering and further analysis of α- and β-diversity with the MOTHUR software.
Related Concept Videos
01:29Modern Molecular Taxonomy
834
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
834
01:20Applications of Molecular Taxonomy
705
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
705