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Related Concept Videos

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

6.4K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Conjugated Proteins02:50

Conjugated Proteins

21.1K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
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Related Experiment Video

Updated: Apr 29, 2026

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
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Bionanoconjugation for proteomics applications - An overview.

João Pinto da Costa1, Rui Oliveira-Silva1, Ana Luísa Daniel-da-Silva2

  • 1Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.

Biotechnology Advances
|May 27, 2014
PubMed
Summary
This summary is machine-generated.

Nanoproteomics offers sensitive, rapid, and high-throughput protein analysis by combining nanotechnology with proteomics. This approach addresses challenges in detecting proteins in complex biological samples for the Human Proteome Project (HUPO).

Keywords:
BioconjugatesNanoparticles and nanomaterialsNanoproteomics

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Area of Science:

  • Proteomics
  • Nanotechnology
  • Bioconjugation

Background:

  • The Human Genome Project highlighted the need for large-scale protein studies (Proteomics).
  • Understanding expressed proteins remains challenging despite genetic information.
  • The Human Proteome Project (HUPO) aims for complete protein characterization.

Purpose of the Study:

  • To review advances in nanolevel bioconjugation for Proteomics.
  • To discuss the application of these advances in protein analysis.
  • To evaluate the prospects and limitations of Nanoproteomics.

Main Methods:

  • Overview of recent advances in bioconjugation at the nanolevel.
  • Focus on nanoparticle-biomolecule hybrid systems.
  • Application of these systems in Proteomics.

Main Results:

  • Nanoproteomics provides short assay times, low sample consumption, and ultralow detection limits.
  • High-throughput capacity is achievable with Nanoproteomics platforms.
  • Biomolecule-nanoparticle hybrids exhibit enhanced or novel features.

Conclusions:

  • Nanoproteomics presents a promising solution for sensitive and efficient protein detection.
  • Further development is needed to overcome current limitations in Nanoproteomics.
  • Nanoproteomics holds significant potential for advancing the goals of the Human Proteome Project.