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A new stationary phase for high performance liquid chromatography: Calix[4]arene derivatized chitosan bonded silica
Jing Lu1, Wenfen Zhang1, Yanhao Zhang1
1College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
Abstract:
In the present paper, a new calix[4]arene derivatized chitosan bonded stationary phase (CCS4) for high performance liquid chromatography (HPLC) was synthesized and characterized. Its chromatographic performance and retention mechanism were evaluated in reversed-phase mode compared with ODS using different solute probes including mono-substituted benzenes, phenols and nucleosides. The results showed that CCS4 stationary phase could provide various interactions with solutes, such as hydrophobic, hydrophilic, π-π, and inclusion interactions. It could perform the mixed-mode separation including RP and HILIC.
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