Related Experiment Video

Updated: Apr 29, 2026

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
08:51

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Published on: June 25, 2015

8.7K

Gene expression profiling of Cecropin B-resistant Haemophilus parasuis

Chunmei Wang1, Fangzhou Chen, Han Hu

  • 1State Key Laboratory of Agricultural Microbiology, Division of Animal Infectious Disease, Huazhong Agricultural University, Wuhan, PR China.

Journal of Molecular Microbiology and Biotechnology
|May 28, 2014
PubMed

Insights

Researchers induced resistance to the antimicrobial peptide cecropin B (CB) in *Haemophilus parasuis*. Resistant strains showed altered cell membranes and gene expression, particularly in ion transport and metabolism, offering insights into antimicrobial resistance mechanisms.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genomics

Background:

  • Antimicrobial peptides (AMPs) are promising alternatives to antibiotics.
  • Microbial resistance to AMPs is emerging, but mechanisms remain unclear.
  • Cecropin B (CB) is a natural AMP effective against Gram-negative bacteria.

Purpose of the Study:

  • To investigate the molecular mechanisms of *Haemophilus parasuis* resistance to cecropin B (CB).
  • To induce and characterize CB-resistant *H. parasuis* strains.
  • To identify genetic changes associated with CB resistance.

Main Methods:

  • Induction of CB resistance in *H. parasuis* SH0165 through serial exposure to CB.
  • Growth curve analysis and transmission electron microscopy (TEM) of resistant strains.
  • Microarray analysis to detect differential gene expression.
  • Validation of microarray data using real-time quantitative reverse transcription PCR (RT-qPCR).

Main Results:

  • CB-resistant *H. parasuis* strains (CBR30 and CBR30-50) were successfully generated.
  • Resistant strains exhibited slower growth rates and smoother cell membranes compared to the wild type.
  • Microarray analysis revealed significant changes in gene expression in resistant strains, affecting 20 Clusters of Orthologous Groups (COGs).
  • Upregulated and downregulated genes in resistant strains were predominantly involved in inorganic ion transport, amino acid transport, and metabolism.

Conclusions:

  • *Haemophilus parasuis* can develop resistance to the antimicrobial peptide cecropin B.
  • CB resistance is associated with alterations in cell membrane structure and significant changes in gene expression profiles.
  • The findings highlight the roles of inorganic ion transport and amino acid metabolism in CB resistance, providing a foundation for understanding and combating AMP resistance.

More Related Videos

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

9.7K
A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae10:18

A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

Published on: April 25, 2015

9.7K

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.4K