Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Primary structure of a lambda Bence Jones protein (Os).

S Ito1, H Matsumoto

  • 1Department of Legal Medicine, Osaka Medical College, Japan.

Bulletin of the Osaka Medical College
|November 1, 1989
PubMed
Summary

The primary structure of human Bence-Jones protein Os was determined, revealing its amino acid sequence. This lambda type protein shows homology to subgroup V lambda I and possesses specific isotypic markers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Establishment of a folliculo-stellate-like cell line from a murine thyrotropic pituitary tumor.

Endocrinology·1992
Same author

Duodenal reflux through the pylorus induces gastric adenocarcinoma in the rat.

Carcinogenesis·1992
Same author

HIV-1 reverse transcriptase inhibitor from Phyllanthus niruri.

AIDS research and human retroviruses·1992
Same author

Charcot-Marie-Tooth disease with diaphragmatic weakness.

Internal medicine (Tokyo, Japan)·1992
Same author

[Pathophysiology and treatment of reflex sympathetic dystrophy].

No to shinkei = Brain and nerve·1992
Same author

Reactivation of rat cytomegalovirus in lung allografts: an experimental and immunohistochemical study in rats.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·1992

Area of Science:

  • Immunology
  • Proteomics
  • Biochemistry

Background:

  • Bence-Jones proteins are monoclonal immunoglobulin light chains.
  • Understanding their primary structure is crucial for diagnosing and studying plasma cell disorders.

Purpose of the Study:

  • To determine the complete amino acid sequence of a human lambda type Bence-Jones protein, designated Os.
  • To analyze the sequence for homology with known lambda chains and identify isotypic markers.

Main Methods:

  • Complete reduction and aminoethylation of the protein.
  • Tryptic digestion and isolation of peptides.
  • Amino acid sequencing and composition analysis.
  • Peptide mapping and sequence alignment based on homology.

Main Results:

  • Nineteen tryptic peptides were isolated, with 10 fully sequenced.
  • The complete amino acid sequence of 213 residues was elucidated.
  • The variable region (109 residues) showed homology to subgroup V lambda I.
  • The constant region indicated specific isotypic markers (Mcg(+), Kern(+), Oz(-)) with a unique residue at position 164.

Conclusions:

  • The primary structure of Bence-Jones protein Os was successfully determined.
  • Protein Os shares sequence homology with other lambda I proteins.
  • The identified isotypic markers provide insights into the protein's genetic and functional characteristics.

Related Experiment Videos