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Label-free fluorescence polarization detection of pyrophosphate based on 0D/1D fast transformation of CdTe
1Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China. zhucq@mail.ahnu.edu.cn.
Abstract:
A novel and label-free fluorescence polarization (FP) method for the determination of pyrophosphate (PPi) is developed based on the change in FP signals during fast reversible transformation between CdTe zero-dimensional (0D) nanocrystals (NCs) and one-dimensional (1D) nanorods (NRs) induced by addition of PPi. Under optimum conditions, the FP ratio was linearly proportional to the logarithm of the concentration of PPi between 2.0 × 10(-5) and 1.0 × 10(-9) M with a detection limit of 8.0 × 10(-10) M. The developed method, with high signal selectivity and stability, was successfully applied to the detection of PPi in human urine samples.
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