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Published on: October 14, 2022
Amniotic membrane as a biological dressing in infected wound healing in rabbits
Ian Göedert Leite Duarte1, Ivana Duval-Araujo2
1Medical School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Purpose:
To investigate amniotic membrane as a biological dressing in infected wound healing in rabbits.
Methods:
The use of preserved amniotic membranes (AMs) was examined using 15 rabbits with experimentally induced wound infections on their backs. Healing was histologically evaluated during different phases including inflammation, granulation, epithelialization, and fibroplasia. The animals were distributed into three groups for histological study at seven, 14, 21, and 28 days post-wound induction. Group A did not receive treatment: the wound was left exposed and dry; Group B received a daily exposure treatment with collagenase; and Group C received one AM, which also remained exposed.
Results:
A marked reduction of the inflammatory phase was observed in Group C at 21 days, and the granulation phase of this healing increased at 14 days. Epithelialization was similar among the three groups, and fibroplasia was more pronounced in Group C at 14 days. Furthermore, gradual collagen organization also began for the animals in Group B at 14 days.
Conclusion:
The amniotic membrane did not significantly alter the inflammation, epithelialization, or fibroplasia phases but did increase angiogenesis up to Day 14 compared with the dry dressing and collagenase treatments.
Insights
Amniotic membrane (AM) dressings accelerated wound healing in rabbits by promoting angiogenesis, a key factor in tissue repair. This biological dressing showed potential in managing infected wounds.
Area of Science:
- Regenerative Medicine
- Wound Healing Research
- Biological Dressings
Background:
- Infected wounds pose significant clinical challenges.
- Biological dressings offer potential for enhanced wound repair.
- Amniotic membrane (AM) is a promising biomaterial for wound management.
Purpose of the Study:
- To evaluate the efficacy of amniotic membrane (AM) as a biological dressing for infected wound healing in rabbits.
- To compare AM treatment with dry dressing and collagenase treatments.
Main Methods:
- Experimentally induced wound infections in 15 rabbits.
- Histological evaluation of healing phases (inflammation, granulation, epithelialization, fibroplasia) at 7, 14, 21, and 28 days.
- Three treatment groups: no treatment (dry), collagenase, and AM.
Main Results:
- Amniotic membrane (AM) treatment showed increased granulation at 14 days and more pronounced fibroplasia at 14 days.
- AM significantly increased angiogenesis up to Day 14 compared to controls.
- No significant alterations in inflammation or epithelialization phases were observed with AM.
Conclusions:
- Amniotic membrane (AM) enhances angiogenesis during the early stages of wound healing.
- AM demonstrates potential as an effective biological dressing for infected wounds.
- Further research can explore AM's role in various wound types and clinical settings.
