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Surface-modified bacterial nanofibrillar PHB scaffolds for bladder tissue repair
Zeynep Karahaliloğlu1, Murat Demirbilek1, Mesut Şam2
1a Division of Nanotechnology and Nanomedicine, Hacettepe University , Beytepe , Ankara , Turkey.
Artificial Cells, Nanomedicine, and Biotechnology
|May 28, 2014
Summary
Surface-modified polyhydroxybutyrate (PHB) nanofibrous scaffolds show promise for bladder reconstruction by inhibiting stone formation and promoting cell growth. This study investigated PHB graft feasibility for enhanced urothelial regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Urology
Background:
- Polyhydroxybutyrate (PHB) is a biodegradable polymer with potential for tissue engineering.
- Bladder reconstruction requires biocompatible materials that promote urothelial healing and prevent complications like stone formation.
- Surface modification techniques can enhance the properties of biomaterials for specific applications.
Purpose of the Study:
- To investigate the in vitro feasibility of surface-modified bacterial nanofibrous poly[(R)-3-hydroxybutyrate] (PHB) grafts for bladder reconstruction.
- To evaluate the effect of plasma modification (PEG or EDA) on PHB scaffold properties, including wettability and calcium oxalate deposition.
- To assess the impact of modified PHB scaffolds on uroepithelial cell viability and proliferation.
Main Methods:
- Electrospinning of bacterial PHB to create nanofibrous scaffolds.
- Surface modification of PHB scaffolds using radio frequency glow discharge with polyethylene glycol (PEG) or ethylenediamine (EDA).
- Contact angle measurements to assess surface wettability.
- In vitro assessment of calcium oxalate stone deposition.
- In vitro evaluation of uroepithelial cell viability and proliferation on modified scaffolds.
Main Results:
- Plasma modification significantly reduced the contact angle of PHB scaffolds, indicating increased wettability.
- EDA-modified PHB scaffolds exhibited reduced calcium oxalate stone deposition compared to non-modified scaffolds.
- Surface-modified PHB scaffolds demonstrated enhanced uroepithelial cell viability and proliferation.
- The modified PHB scaffolds showed potential in inhibiting calcium oxalate crystal growth.
Conclusions:
- Surface modification of bacterial nanofibrous PHB scaffolds using plasma treatment is a feasible approach for bladder reconstruction applications.
- Modified PHB scaffolds exhibit anti-lithogenic properties and support urothelial cell growth, suggesting their potential for improving bladder tissue regeneration.
- These findings highlight the potential of engineered PHB biomaterials in urological tissue engineering and regenerative medicine.
