Isolation and partial characterization of the cell membrane of Walker-256 carcinosarcoma ascites cells

Cell membranes of carcinosarcoma-256 ascites cells were isolated by the Zn++ method. Neutral sugars, sialic acid, fucose and N-Acetylhexosamines were determined in the cell membrane fraction. Cells were agglutinated by wheat germ agglutinin, Concanavalin A, and the lectins from Phaseolus vulgaris and Glycine max. No agglutination was observed with peanut lectin (Arachis hypogaea). Gel electrophoresis under dissociating conditions revealed more than 40 bands in the membrane fraction after silver staining. Only 2 bands were evident with PAS staining.