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Updated: Apr 29, 2026
Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization
Published on: August 4, 2023
Laboratory evaluation of different agar media for isolation of carbapenem-resistant Acinetobacter spp
J Moran-Gilad1, A Adler, D Schwartz
1Public Health Services, Ministry of Health, 39 Yermiyahu Street, Jerusalem, Israel, giladko@post.bgu.ac.il.
Abstract:
The optimal method for surveillance of carbapenem-resistant Acinetobacter spp. (CRAB) is unknown. A collection of CRAB strains (n = 42), carbapenem-susceptible strains (CSAB), and non-Acinetobacter strains (n = 18) was used to evaluate six laboratory surveillance methods: MacConkey (MAC), MAC + 1 μg/ml imipenem (MAC-IPM), minimal salts agar + 1 % acetate (MSA), MSA with IPM disk (MSA-IPM), CHROMagarKPC, and CHROMagar Acinetobacter with CR102 (CHROMAcineto). CHROMAcineto was 100 % sensitive and specific. CHROMagarKPC and MAC-IPM were highly sensitive (>95 %), but their specificity was substantially hampered by the breakthrough growth of CSAB. MSA was unsuitable for CRAB detection. CHROMAcineto is a promising medium for CRAB detection and warrants further clinical evaluation.
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