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Updated: Apr 29, 2026
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Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
Published on: November 19, 2019
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Transcriptome profiling of prostate tumor and matched normal samples by RNA-Seq
1Department of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. jacky_zw2002@hotmail.com.
Summary
RNA-Sequencing (RNA-Seq) identified differentially expressed genes and a lincRNA in prostate tumors. This lincRNA, antisense to CAND1, is repressed in tumors, suggesting a potential role in cancer progression.
Area of Science:
- Oncology
- Genomics
- Bioinformatics
Background:
- RNA-Sequencing (RNA-Seq) offers high-resolution gene expression analysis in cancer research.
- RNA-Seq surpasses cDNA microarray in sensitivity and reduced noise.
Purpose of the Study:
- Identify protein-coding genes and long non-coding RNAs (lincRNAs) with differential expression between tumor and normal tissues.
- Investigate potential biomarkers for prostate cancer.
Main Methods:
- Analyzed transcriptome datasets from 10 human prostate tumor and matched normal tissues.
- Utilized RNA-Sequencing technology for gene expression profiling.
- Aligned short reads to human RefSeq genes and lincRNAs.
Main Results:
- Identified 10 RefSeq genes differentially expressed (p < 0.05) between tumor and normal samples.
- Discovered a lincRNA antisense to CAND1, repressed in prostate tumors (p < 0.1).
- Observed an inverse expression pattern between CAND1 and its antisense lincRNA in tumor versus normal tissues.
Conclusions:
- RNA-Sequencing is valuable for cancer biomarker development.
- The identified lincRNA and its interaction with CAND1 warrant further functional investigation in prostate cancer.
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