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Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
Published on: August 16, 2019
Scanning electron microscopy of eggs of Georgecraigius fluviatilis (Lutz) (Diptera: Culicidae, Aedini)
Juliana Soares Sarmento1, Carlos Brisola Marcondes2, Jeronimo Alencar3
1Sector of Medical and Forensic Entomology, Laboratory of Vectors of Leishmaniases, Oswaldo Cruz Institute (Fiocruz), Av. Brasil 4365, CEP 21040-360, Manguinhos, Rio de Janeiro, Brazil. Postgraduate Program on Animal Biology, Institute of Biology, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil;
Abstract:
Scanning Electron Microscopy was used to describe the eggs of Georgecraigius fluviatilis (Lutz). Length is 722.8±39.6 µm and width is 177.1±9.8 µm. Diameter of the micropylar disk, surrounded by an irregular flattened collar, is 28 µm. The outer chorionic sculpture consists of cells of irregular shapes, containing tubercles with pitted surface. In the ventral region, tubercles of several diameters are irregularly distributed in chorionic cells, while in the dorsal region one larger tubercle is surrounded by several smaller ones. The eggs appear to lack structures for adhesion, certainly unnecessary due to the habit of laying eggs separately on water surfaces.
