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Updated: Apr 28, 2026

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Published on: July 23, 2010

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Large scale RNAi reveals the requirement of nuclear envelope breakdown for nuclear import of human papillomaviruses

Inci Aydin1, Susanne Weber1, Berend Snijder2

  • 1Emmy-Noether Group: Virus Endocytosis, Institutes of Molecular Virology and Medical Biochemistry, ZMBE, University of Münster, Münster, Germany; Cluster of Excellence EXC1003, Cells in Motion, Münster, Germany.

Plos Pathogens
|May 31, 2014
PubMed

Insights

Human papillomavirus type 16 (HPV16) requires nuclear envelope breakdown during mitosis for viral DNA to enter the nucleus. This study identified key host cell factors essential for HPV16 infection.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Human papillomavirus type 16 (HPV16) is a significant human pathogen.
  • Understanding HPV16 infection mechanisms is crucial for developing antiviral strategies.

Purpose of the Study:

  • To identify host cell factors essential for HPV16 infection using a high-throughput RNAi screen.
  • To elucidate the mechanism of viral DNA import into the host cell nucleus.

Main Methods:

  • A two-step, high-throughput RNAi silencing screen was employed to identify host factors.
  • Analysis of validated hits and viral DNA localization during the cell cycle.

Main Results:

  • A cluster of mitotic genes was implicated in HPV16 infection.
  • HPV16 viral DNA (vDNA) is endocytosed and uncoated in interphase but fails nuclear import.
  • vDNA and L2 associate with host chromatin during mitosis, requiring nuclear envelope breakdown for nucleoplasmic access.

Conclusions:

  • HPV16 infection necessitates nuclear envelope breakdown during mitosis for viral DNA entry.
  • Identified host cell factors provide a resource for future studies on HPV16-host interactions.

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