Related Experiment Video
Updated: Apr 28, 2026
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
The granulocyte macrophage-colony stimulating factor surface modified MB49 bladder cancer stem cells vaccine against
Yong-tong Zhu1, Zhen Zhao1, Xin-yang Fu1
1Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Abstract:
The MB49 bladder cancer cell vaccine was effective against bladder cancer in the mice model in previous studies. However, part of the tumors regrew as the vaccine could not eliminate the cancer stem cells (CSCs). MB49 bladder cancer stem cells (MCSCs) were isolated by a combination of the limited dilution method and the serum free culture medium method. MCSCs possessed higher expression of CD133, CD44, OCT4, NANOG, and ABCG2, the ability of differentiation, higher proliferative abilities, lower susceptibility to chemotherapy, greater migration in vitro, and stronger tumorigenic abilities in vivo. Then streptavidin-mouse granulocyte macrophage-colony stimulating factor (SA-mGM-CSF) MCSCs vaccine was prepared. SA-mGM-CSF MCSCs vaccine extended the survival of the mice and inhibited the growth of tumor in protective, therapeutic, memorial and specific immune response experiments. The level of immunoglobulin G and the ratio of dendritic cells and CD4(+) and CD8(+) T cells were highest in the experimental group when compared to those in other four control groups, as well as for the cytotoxicity assay. We demonstrated that SA-mGM-CSF MCSCs vaccine induces an antitumor immune response to metastatic bladder cancer.
Insights
A novel streptavidin-mouse granulocyte macrophage-colony stimulating factor (SA-mGM-CSF) bladder cancer stem cell (CSC) vaccine effectively targets and eliminates cancer stem cells, significantly improving survival in a mouse model.
Area of Science:
- Oncology
- Immunology
- Cancer Stem Cell Biology
Background:
- Previous MB49 bladder cancer vaccines showed efficacy but failed to eradicate cancer stem cells (CSCs), leading to tumor regrowth.
- Cancer stem cells (CSCs) are implicated in tumor recurrence and metastasis due to their unique properties.
Purpose of the Study:
- To isolate and characterize MB49 bladder cancer stem cells (MCSCs).
- To develop and evaluate a novel streptavidin-mouse granulocyte macrophage-colony stimulating factor (SA-mGM-CSF) MCSC vaccine for its efficacy against metastatic bladder cancer.
Main Methods:
- MCSCs were isolated using limited dilution and serum-free culture methods.
- Characterization of MCSCs included analysis of CSC markers (CD133, CD44, OCT4, NANOG, ABCG2), differentiation, proliferation, chemotherapy susceptibility, migration, and tumorigenicity.
- A SA-mGM-CSF MCSC vaccine was prepared and tested in various experimental settings (protective, therapeutic, memorial).
Main Results:
- Isolated MCSCs exhibited enhanced expression of CSC markers and demonstrated increased tumorigenic and migratory potential.
- The SA-mGM-CSF MCSC vaccine significantly extended survival and inhibited tumor growth in mice.
- Vaccination led to elevated levels of immunoglobulin G and increased ratios of dendritic cells and CD4+/CD8+ T cells, indicating a robust antitumor immune response.
Conclusions:
- The SA-mGM-CSF MCSC vaccine is effective in eliminating bladder cancer stem cells.
- This novel vaccine formulation induces a potent antitumor immune response against metastatic bladder cancer, offering a promising therapeutic strategy.
More Related Videos
11:02Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
Published on: October 30, 2013
06:12Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model
Published on: May 29, 2015
Related Concept Videos
01:30Cancer Vaccines
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
01:19Mesenchymal Stem Cells