Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

3.7K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.7K
Protein Networks02:26

Protein Networks

1.8K
1.8K
Next-generation Sequencing03:00

Next-generation Sequencing

87.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.9K
RNA-seq03:21

RNA-seq

9.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.4K
Sequence Networks of Rotating Machines01:24

Sequence Networks of Rotating Machines

592
A Y-connected synchronous generator, grounded through a neutral impedance, is designed to produce balanced internal phase voltages with only positive-sequence components. The generator's sequence networks include a source voltage that is exclusively in the positive-sequence network. The sequence components of line-to-ground voltages at the generator terminals illustrate this configuration.
Zero-sequence current induces a voltage drop across the generator's neutral impedance and other...
592
DNA Microarrays02:34

DNA Microarrays

16.7K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
16.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

What's past is prologue: epigenetic memory links transient inflammation to future disease.

Signal transduction and targeted therapy·2026
Same author

Urea-cycle control of fibroblast energetics after myocardial infarction.

Cardiovascular research·2026
Same author

Rezatapopt and the Return of Targetable p53.

MedComm·2026
Same author

Predictive neural networks accelerate identification of mechanistically validated small-molecule modulators of TLR7 signaling.

Biochemical pharmacology·2026
Same author

Mechanism of Mutation in G Protein-Gated Inwardly Rectifying K<sup>+</sup> Channel in Familial Hyperaldosteronism-Type III: Residue Fluctuations and Conformational Instability.

Molecules (Basel, Switzerland)·2026
Same author

The significance of the ketolytic gene OXCT1 in metabolism, intracellular signaling and disease development.

Cellular and molecular life sciences : CMLS·2026

Related Experiment Video

Updated: Apr 28, 2026

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

7.7K

Deep sequencing reveals novel Set7 networks.

Samuel T Keating1, Mark Ziemann, Jun Okabe

  • 1Epigenetics in Human Health and Disease Laboratory, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, Melbourne, VIC, 3004, Australia.

Cellular and Molecular Life Sciences : CMLS
|May 31, 2014
PubMed
Summary
This summary is machine-generated.

Set7 lysine methyltransferase regulates gene expression in vascular endothelial cells, impacting development and inflammation. Its role extends beyond histone modification, potentially involving transcription factor methylation.

More Related Videos

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
09:14

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

6.7K
JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

2.7K

Related Experiment Videos

Last Updated: Apr 28, 2026

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

7.7K
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
09:14

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

6.7K
JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

2.7K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Vascular Biology

Background:

  • Methyl-dependent transcription regulation involves histones and transcription factors.
  • Set7 lysine methyltransferase is linked to inflammation in vascular endothelial cells.
  • Set7's genome-wide regulatory roles and non-histone substrates require investigation.

Purpose of the Study:

  • To investigate the genome-wide regulatory roles of Set7 in vascular endothelial cells.
  • To explore Set7's function beyond histone H3 lysine 4 monomethylation (H3K4m1).
  • To identify Set7's downstream targets and regulatory mechanisms.

Main Methods:

  • Messenger RNA sequencing (mRNA-Seq) to analyze gene expression changes.
  • Chromatin immunoprecipitation sequencing (ChIP-Seq) for H3K4m1 profiling.
  • Transcription factor network analysis to identify regulatory relationships.

Main Results:

  • Set7 knockdown deregulated over 8,000 genes, affecting developmental and inflammatory pathways.
  • Loss of H3K4m1 at regulatory elements had a subtle effect on gene expression.
  • Six known Set7 substrates were implicated; other transcription factor targets are predicted.

Conclusions:

  • Set7 regulates developmental pathways and responses to stimuli in vascular endothelial cells.
  • Set7-dependent gene expression changes may involve transcription factor methylation, independent of H3K4m1.
  • The study presents a method for predicting substrates of broad-substrate modifiers.