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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
RNA-guided genome editing in Drosophila with the purified Cas9 protein
Jeong-Soo Lee1, Su-Jin Kwak2, Jungeun Kim3
1Neurophysiology Research Group, Bionano Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea Functional Genomics Department, Korea University of Science and Technology (KUST), Daejeon 305-333, Korea.
Abstract:
We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
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