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Related Experiment Video

Updated: Apr 28, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
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Published on: May 3, 2011

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Confocal photoacoustic microscopy using a single multifunctional lens.

Lei Xi, Chaolong Song, Huabei Jiang

    Optics Letters
    |May 31, 2014
    PubMed
    Summary

    This study introduces a compact photoacoustic microscopy system using a single liquid lens for enhanced sensitivity and resolution. This innovation simplifies confocal configurations for advanced biomedical imaging applications.

    Area of Science:

    • Biomedical Imaging
    • Optics and Acoustics

    Background:

    • Photoacoustic microscopy (PAM) is a rapidly advancing biomedical imaging technique.
    • Confocal strategies enhance PAM sensitivity but often require bulky, complex systems with separate optical and acoustic focusing elements.

    Purpose of the Study:

    • To develop a simplified and compact confocal configuration for optical-resolution PAM (ORPAM).
    • To demonstrate the efficacy of a single liquid lens in achieving simultaneous acoustic and optical focusing.

    Main Methods:

    • Utilized a single liquid lens with a numerical aperture of 0.43 in an ORPAM system.
    • Integrated acoustic and optical focusing using the single liquid lens to achieve confocal operation.

    Main Results:

    • Achieved a system resolution of 4.8 μm.

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  • Demonstrated significantly improved acoustic detection sensitivity.
  • Successfully applied the compact ORPAM system for in vivo imaging of rat ear vasculature.

    • Conclusions:

    • A single liquid lens can effectively create an acoustic and optical confocal configuration for ORPAM.
    • This approach leads to a more compact and simpler ORPAM system with enhanced performance.
    • The developed system is suitable for in vivo biomedical imaging applications.