An in-vitro evaluation of effect of EDTAC on root dentin with respect to time
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Updated: Apr 28, 2026

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Published on: August 6, 2019
Sumeet Darda1, Kunal Madria2, Rohan Jamenis2
1Department of Conservative Dentistry & Endodontics, Yogita Dental College & Hospital, Khed, Ratnagiri, Maharashtra, India.
Background:
The present study was devised to evaluate the effects of 17% EDTAC on smear layer removal and on the dentin structure after irrigation with 1 minute and 10 minutes.
Materials & Methods:
One hundred extracted mandibular molars with two separate mesial canals were selected; mesiobuccal canal was instrumented to size 30 file with crown down technique. One half of each root (either mesial or distal) was randomly selected and prepared for scanning electron microscopic (SEM) evaluation that was then cut longitudinally into two equal segments. Using 10 ml of 17% EDTA solution, halves belonging to the same root were irrigated for 1 and 10 min, respectively. All specimens were subjected to irrigation with 10 ml of 5% NaOCl. Then all the specimens were prepared for SEM evaluation.
Results:
The results showed that 1 min EDTA irrigation is effective in removing the smear layer. However a 10-min application of EDTA caused excessive peritubular and intertubular dentinal erosion.
Conclusion:
Therefore we suggest that this procedure should not be prolonged >1 min during endodontic treatment. How to cite the article: Darda S, Madria K, Jamenis R, Heda A, Khanna A, Sardar L. An in-vitro evaluation of effect of EDTAC on root dentin with respect to time. J Int Oral Health 2014;6(2):22-7.
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