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Effect of Furin inhibitor on lung adenocarcinoma cell growth and metastasis
Yong-Chao Ma1, Wen-Juan Fan1, Shu-Mei Rao1
1Luo He Medical Colledge, Daxue Road, #148, Luohe City, Henan Province 462002, P.R. China.
Background:
To investigate the mechanisms of lung adenocarcinoma cell metastasis and provide a theoretical basis for the in-depth study of lung adenocarcinoma.
Methods:
A549 cells are incubated with different concentrations of Furin inhibitor for indicated times. The proliferation and migration were confirmed with MTT, colony formation, wound Healing and Transwell assayes. Hochest 33342 / PI double staining was used to detect apoptosis. Cell migration and apoptosis associated proteins were analysed by enzyme-linked immunosorbent assay (ELISA) and western blot.
Results:
We have found that Furin inhibitor play a significant role in inhibition A549 cell growth. And we also found cell migration was inhibited significantly upon Furin inhibitor treatment.
Conclusion:
The proliferration and migration of A549 cell were inhibited by Furin inbitor through down-regulation the expression of migration and apoptosis related proteins.
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