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Is less more? Lessons from aptamer selection strategies.

Zsuzsanna Szeitner1, Judit András1, Róbert E Gyurcsányi2

  • 1Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47, H-1094 Budapest, Hungary.

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Summary

Aptamers offer advantages over antibodies in bioassays, but their selection must prioritize target binding and analytical application for success. Careful consideration during aptamer selection enhances their real-world utility.

Keywords:
Aptamer assayAptamer selectionBioanalysisCounter-selectionSELEX

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry

Background:

  • Aptamers are advantageous alternatives to antibodies in bioaffinity assays due to in vitro selection and chemical synthesis.
  • Current aptamer assay development often overlooks the crucial initial selection phase, limiting their practical application.
  • Analytical chemists have focused on assay methods rather than optimizing aptamer selection for specific analytical needs.

Purpose of the Study:

  • To provide a subjective overview of trends in aptamer development.
  • To highlight critical factors for aptamer selection in analytical applications.
  • To emphasize the importance of integrating analytical considerations early in the aptamer development process.

Main Methods:

  • Review of current trends in aptamer selection strategies.
  • Discussion of factors influencing aptamer functionality in analytical assays.
  • Analysis of the impact of sample matrix considerations during aptamer selection.

Main Results:

  • Aptamer selection is paramount for achieving high target affinity and specificity.
  • Incorporating sample matrix information during counter-selection improves aptamer applicability.
  • Early consideration of analytical use enhances the success rate of aptamer-based assays.

Conclusions:

  • The selection process for aptamers must be analytically driven to maximize their potential.
  • Optimizing aptamer selection is key to overcoming limitations in bioaffinity assays.
  • This review emphasizes strategic aptamer selection for robust analytical applications.