Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of dental diseases with pulmonary outcomes in a multicenter cohort of adolescents and young adults with CF.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2026
Same author

Unlabeled kratom alkaloids detected in products marketed as kava.

Clinical toxicology (Philadelphia, Pa.)·2026
Same author

Charleson and Elixhauser Comorbidity Indices in Predicting Mortality Among Young and Old Cervical Cancer Patients.

Cancer medicine·2026
Same author

Phase 2 Futility Trials in Alzheimer Disease and Mild Cognitive Impairment: A Cohort Analysis of the ADNI Data Set.

Neurology·2026
Same author

Application of the 2024 McDonald Criteria in Individuals With Nonspecific Symptoms or Incidental Imaging Findings in a Multicenter Study.

Neurology·2026
Same author

Efficacy and safety of ocrelizumab in primary progressive multiple sclerosis, including older patients and those with more advanced disease (ORATORIO-HAND): a multicentre, double-blind, randomised, placebo-controlled, phase 3b study.

Lancet (London, England)·2026

Related Experiment Video

Updated: Apr 28, 2026

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
12:06

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Published on: October 23, 2017

7.4K

Mutans streptococci enumeration and genotype selection using different bacitracin-containing media.

Stephanie S Momeni1, Paul Patrick1, Howard W Wiener2

  • 1Department of Pediatric Dentistry, School of Dentistry, University of Alabama at Birmingham, SOD 304, 1720 2nd Avenue South, Birmingham, AL 35294-0007, USA.

Journal of Microbiological Methods
|June 1, 2014
PubMed
Summary

This study compared five bacitracin-containing media for isolating mutans streptococci (MS), key bacteria in dental caries. All tested media showed similar effectiveness in enumerating MS, with no significant differences in bacterial counts or genotype recovery.

Keywords:
GenotypesSelective mediaStreptococcus mutansStreptococcus sobrinusTotal streptococcirep-PCR

More Related Videos

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

15.8K
Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes
03:53

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Published on: April 19, 2024

1.3K

Related Experiment Videos

Last Updated: Apr 28, 2026

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
12:06

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Published on: October 23, 2017

7.4K
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

15.8K
Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes
03:53

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Published on: April 19, 2024

1.3K

Area of Science:

  • Microbiology
  • Dental Research
  • Epidemiology

Background:

  • Mutans streptococci (MS) are primary etiological agents of dental caries.
  • Accurate isolation and enumeration of MS are crucial for caries research and clinical assessment.
  • Existing selective media may inhibit MS growth, affecting strain selection and counts.

Purpose of the Study:

  • To quantitatively compare the efficacy of five bacitracin-containing culture media for isolating MS.
  • To evaluate the impact of different media on MS enumeration and genotype recovery.
  • To assess the performance of MSB, MSKB, GTSB, TYS20B, and TYCSB media.

Main Methods:

  • Quantitative evaluation of five selective media using standard plate counts.
  • Testing with prototype MS strains (S. mutans, S. sobrinus) and clinical isolates from children's saliva.
  • Confirmation of S. mutans using real-time PCR and genotype analysis via rep-PCR.

Main Results:

  • All five media exhibited variable but not significantly different MS counts.
  • Prototype MS strains grew well on all media; clinical isolates showed count variability.
  • MSB allowed for increased non-MS growth compared to other media.
  • All media consistently identified a predominant MS genotype, with no inhibition of minor genotype recovery.

Conclusions:

  • The tested bacitracin-containing media demonstrate comparable efficacy for MS isolation and enumeration.
  • No single medium significantly outperformed others in recovering MS or their genotypes.
  • Further research may be needed to optimize media for selective MS recovery and minimize non-target organism growth.