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Updated: Apr 28, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping
Published on: January 8, 2008
Isolation and characterization of novel microsatellite markers for the northern mauxia shrimp, Acetes chinensis,
Jung-Ha Kang1, Hyun-Sook Kang1, Eun-Soo Noh1
1Biotechnology Research Division, NFRDI, Busan 619-705, Republic of Korea.
Abstract:
Among the 14 species in the Acetes genus, Acetes japonicus and Acetes chinensis, are the only and also abundant two species around the Korean Peninsula, and are used in traditional recipes. These species are of great economic importance, but little is known about their population genetics, despite the fact that information of this kind is important for stock assessment, fisheries management and identification of origin. A total of 9 microsatellite (MS) markers for A. chinensis were developed using pyrosequencing techniques. Polymorphisms of these markers were evaluated in 96 wild individuals collected from the Yellow Sea off the coast of Korea. A total of 133 alleles were detected at nine loci, with a cross-species transferability of 56% with A. japonicus. These markers will facilitate assessment of population genetic diversity in the genus Acetes.

