Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

870
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
870
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.7K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.7K
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

1.8K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
1.8K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.3K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.3K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.3K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.3K
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

3.4K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Lipid-Conjugation Strategy for Intracellular Reactive Oxygen Species Control in Hepatic Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

Enhancing spin coherence times in solid-state NMR using tailored heteronuclear spin decoupling.

Progress in nuclear magnetic resonance spectroscopy·2026
Same author

Flexible Organic Radical Cocrystal With 94% Photothermal Conversion Efficiency.

Angewandte Chemie (International ed. in English)·2026
Same author

Accordion-like tuning of composite pulse dipolar recoupling in solid-state NMR.

The Journal of chemical physics·2026
Same author

Water-generated dangling linkers in a metal-organic framework.

Nature communications·2026
Same author

Orientation Selection in Proton-Detected Magic-Angle Spinning Torsion Angle Experiments.

The journal of physical chemistry. A·2026

Related Experiment Video

Updated: Apr 28, 2026

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

4.8K

r TPPM: towards improving solid-state NMR two-pulse phase-modulation heteronuclear dipolar decoupling sequence by

Asif Equbal1, Subhradip Paul2, Venus Singh Mithu3

  • 1Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience, Center (iNANO) and Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|June 1, 2014
PubMed
Summary
This summary is machine-generated.

A new refocused Two-Pulse Phase-Modulation (TPPM) method, r TPPM, enhances heteronuclear decoupling. This robust technique improves experimental outcomes in solid-state NMR spectroscopy.

Keywords:
-TPPMHeteronuclear dipolar decouplingMagic-angle spinningSPINALSolid-state NMRTPPMr TPPM

More Related Videos

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.2K
Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
13:16

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

1.6K

Related Experiment Videos

Last Updated: Apr 28, 2026

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

4.8K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.2K
Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
13:16

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

1.6K

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) spectroscopy
  • Advanced pulse sequence development
  • Materials characterization

Background:

  • Heteronuclear decoupling is crucial for high-resolution solid-state NMR.
  • The Two-Pulse Phase-Modulation (TPPM) is an established heteronuclear decoupling technique.
  • Existing methods can be sensitive to experimental parameter variations.

Purpose of the Study:

  • To introduce a modified TPPM sequence, termed r TPPM.
  • To evaluate the performance and robustness of r TPPM compared to standard TPPM.
  • To assess the applicability of r TPPM across different magic-angle spinning frequencies.

Main Methods:

  • Development and implementation of the refocused TPPM (r TPPM) pulse sequence.
  • Computational simulations to compare TPPM and r TPPM under varying experimental conditions.
  • Experimental validation using U-(13)C-glycine and U-(13)C-L-histidine.HCl.H2O samples.

Main Results:

  • The r TPPM sequence demonstrates improved decoupling efficiency.
  • r TPPM exhibits enhanced robustness against variations in experimental parameters like RF field strength and pulse timing.
  • Simulations and experimental results show comparable or superior performance of r TPPM at low to moderate magic-angle spinning frequencies.

Conclusions:

  • The r TPPM sequence offers a significant improvement over standard TPPM for heteronuclear decoupling in solid-state NMR.
  • Its increased robustness simplifies experimental setup and enhances reliability.
  • r TPPM is a valuable advancement for researchers utilizing solid-state NMR for structural and dynamic studies.