A novel VHH antibody targeting the B cell-activating factor for B-cell lymphoma
Wen Wu1, Shenghua Li2, Weijing Zhang3
1Department of Lymphoma, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China. wuwen1981@163.com; Department of Blood Transfusion, Jinan Military General Hospital, Jinan 250031, China.
Objective:
To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody.
Methods:
Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA).
Results:
We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro.
Conclusion:
The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.
Related Concept Videos
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Hybridoma Technology
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Humoral Immune Responses
Special Features of Adaptive Immunity
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...


