Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.8K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.6K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.6K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Well-ST-seq: Cost-Effective and Near-Cellular Spatial Transcriptomics Using Deterministic Barcoded Bead Arrays.

Analytical chemistry·2026
Same author

Corrigendum to "Biochemical characterization and identification of catalytic residues of the thermostable inorganic pyrophosphatase from Thermococcus litoralis" [Int. J. Biol. Macromol. 347 (2026) 150779].

International journal of biological macromolecules·2026
Same author

A SERS-based biosensor for optical monitoring of PARP1 trapping dynamics and high-throughput screening of modulators.

Journal of nanobiotechnology·2026
Same author

Biochemical characterization and identification of catalytic residues of the thermostable inorganic pyrophosphatase from Thermococcus litoralis.

International journal of biological macromolecules·2026
Same author

Live attenuated influenza vaccine with low proportions of defective interfering particles elicits robust immunogenicity and cross-protection.

Nature communications·2025
Same author

IL-2 and IFN-γ Secretion of Activated Jurkat T Cells <i>via</i> a Microdroplet-SERS based Single-Cell Immunoassay (Drop-SCIA).

Analytical chemistry·2025

Related Experiment Video

Updated: Apr 28, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

858

Note: Raman microspectroscopy integrated with fluorescence and dark field imaging.

Haibo Li1, Hailong Wang1, Dianshuai Huang2

  • 1State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, People's Republic of China.

The Review of Scientific Instruments
|June 2, 2014
PubMed
Summary

A novel integrated Raman platform combines fluorescence and dark field microscopy for precise in situ detection. This advanced system enhances research on biological tissues and subwavelength samples.

More Related Videos

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

12.6K
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.7K

Related Experiment Videos

Last Updated: Apr 28, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

858
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

12.6K
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.7K

Area of Science:

  • Spectroscopy
  • Microscopy
  • Nanotechnology

Background:

  • Raman spectroscopy offers valuable chemical information but requires precise sample targeting.
  • Conventional Raman systems lack integrated imaging for locating specific micro regions.
  • Analyzing biological tissues and nanoparticles with Raman spectroscopy presents targeting challenges.

Purpose of the Study:

  • To develop an integrated Raman detection platform combining fluorescence and dark field microscopy.
  • To enable in situ Raman detection with enhanced spatial resolution and target identification.
  • To expand the applicability of Raman spectroscopy for analyzing complex biological and nanoscale samples.

Main Methods:

  • Integration of a Raman spectrometer with fluorescence and dark field microscopes.
  • Utilizing fluorescence imaging with labeling techniques for cell and organelle localization.
  • Employing dark field microscopy for the observation and location of nano-sized particles.
  • Performing in situ Raman detection on targeted micro regions identified by imaging modalities.

Main Results:

  • Successful construction of a versatile integrated Raman detection platform.
  • Demonstrated capability to locate specific micro regions, including cells, organelles, and nanoparticles, using combined imaging techniques.
  • Enabled precise in situ Raman analysis facilitated by accurate target identification.
  • Showcased the platform's potential for advanced research applications.

Conclusions:

  • The integrated Raman platform significantly improves target localization for in situ analysis.
  • This system offers a powerful tool for Raman spectroscopy research, particularly in biological and nanoscale fields.
  • The combined microscopy approach overcomes limitations of conventional Raman spectrometers for complex sample analysis.