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[Effect of the peri-implantitis on the biological function of osteoblasts obtained from the mandibles]
Objective:
To study the effect of peri-implantitis inflammatory microenvironment on the biological function of jaw bone osteoblasts.
Methods:
Primary mandible osteoblasts from peri-implantitis and normal tissue were isolated and cultured. Third-generation purified osteoblasts were identified and detected. The proliferative activity of osteoblasts was evaluated through MTT assay. Osteocalcin (OCN), Runx2, and collagen I (Col I) mRNA levels were examined by real-time quantitative polymerase chain reaction. OCN protein levels were determined by Western blot.
Results:
: After 4 d of culture, the proliferative activity of osteoblasts from peri-implantitis became lower than that of normal tissue ( P <0.05). After 7 d of culture, OCN, Runx2, and Col I mRNA expression decreased ( P <0.05). The OCN protein levels also decreased ( P <0.05).
Conclusion:
Peri-implantitis inflammatory microenvironment can decrease the proliferation and differentiation activity of mandible osteoblasts.
