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Updated: Apr 28, 2026
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
An aptamer-based quartz crystal microbalance biosensor for sensitive and selective detection of leukemia cells using
Wenqian Shan1, Yuliang Pan1, Heting Fang1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Abstract:
An aptamer-based quartz crystal microbalance (QCM) biosensor was developed for the selective and sensitive detection of leukemia cells. In this strategy, aminophenylboronic acid-modified gold nanoparticles (APBA-AuNPs) which could bind to cell membrane were used for the labeling of cells followed by silver enhancement, through which significant signal amplification was achieved. Both the QCM and fluorescence microscopy results manifested the selectivity of the sensor designed. A good linear relationship between the frequency response and cell concentration over the range of 2×10(3)-1×10(5)cells/mL was obtained, with a detection limit of 1160cells/mL. This approach provides a simple, rapid, and economical method for leukemia cell analysis which might have great potential for further use.
