Related Experiment Video

Updated: Apr 28, 2026

Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens
13:47

Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens

Published on: May 19, 2020

3.0K

A negative effect of Campylobacter capsule on bacterial interaction with an analogue of a host cell receptor

Sona Rubinchik, Alan M Seddon, Andrey V Karlyshev1

  • 1School of Life Sciences, Kingston University, Faculty of Science, Engineering and Computing, Penrhyn Road, Kingston-upon Thames KT1 2EE, UK. a.karlyshev@kingston.ac.uk.

BMC Microbiology
|June 3, 2014
PubMed
Abstract

Insights

Campylobacter jejuni (C. jejuni) adhesion to host cells is influenced by its capsule and PEB3 adhesin. Capsule production reduces bacterial attachment, and PEB3 is crucial for this interaction, with differential gene regulation observed.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pathogenesis

Background:

  • Campylobacter jejuni (C. jejuni) is a primary cause of bacterial gastroenteritis.
  • Antibiotic resistance in C. jejuni necessitates new therapeutic strategies, such as inhibiting bacterial attachment.
  • The roles of C. jejuni adhesins and its capsule in host cell interaction and pathogenesis require further clarification.

Purpose of the Study:

  • To investigate the role of the C. jejuni capsule in bacterial adhesion to host cells.
  • To elucidate the function of specific adhesins, PEB3 and JlpA, in C. jejuni-host cell interactions.
  • To develop and validate an in vitro model for studying bacterial adhesion.

Main Methods:

  • Development and validation of an in vitro bacterial adhesion assay.
  • Utilizing competitive inhibition, exoglycosidase treatment, and site-directed mutagenesis for assay validation.
  • Analysis of gene expression for capsule production (kpsM) and PEB3 adhesin biosynthesis in liquid culture.

Main Results:

  • The PEB3 adhesin is essential for C. jejuni interaction with host cell receptor analogues.
  • The JlpA adhesin is not required for C. jejuni attachment to the tested receptor analogue.
  • Bacterial capsule production was found to significantly reduce C. jejuni attachment to host cells.

Conclusions:

  • An in vitro model was established to study C. jejuni interaction with host cell receptor analogues.
  • The bacterial capsule interferes with C. jejuni attachment.
  • Differential gene regulation of capsule production (kpsM) and PEB3 adhesin (peb3) was observed, suggesting complex control mechanisms in pathogenesis.

More Related Videos

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

17.6K
Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products05:34

Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products

Published on: February 23, 2024

2.8K

Related Concept Videos

Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
181
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
3.0K
Colonisation of Pathogens01:25

Colonisation of Pathogens

Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...
77
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
6.8K
Inhibitors of Bacterial Protein Synthesis01:25

Inhibitors of Bacterial Protein Synthesis

Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...
102
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
90