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Rehydratable agarose gels: use of low EEO agarose without charged additives
1Section on Macromolecular Analysis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Applied and Theoretical Electrophoresis : the Official Journal of the International Electrophoresis Society
|January 1, 1989
Abstract:
Agarose [SeaKem HGT(P)] gels of 0.5% to 1.2% can be dried at room temperature and rehydrated to 84-85% of their original water content. Mobilities, using the 84-85% rehydrated gels, are indistinguishable from those on the original gels. This suggests that the fiber structure and effective pore size of agarose are independent on the 16% loosely bound (and probably synersed) water.