Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ion retention by immobilized pH matrices.

E Gianazza1, A Osnaghi, L Bontempi

  • 1Department of Biomedical Sciences and Technologies, University of Milano, Italy.

Applied and Theoretical Electrophoresis : the Official Journal of the International Electrophoresis Society
|January 1, 1989
PubMed
Summary

The width of salt fronts in polyacrylamide gel electrophoresis is pH-dependent, wider at neutral pH and for anions. Matrix buffering power significantly impacts front width more than ionic strength.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In silico analysis of patient specific coagulation and flow effects on fibrin clot formation.

Scientific reports·2026
Same author

Effects of immune system status on long-term results of cardiac resynchronization therapy.

Minerva cardioangiologica·2014
Same author

Heterogeneity of storage proteins in maize.

Planta·2014
Same author

MBNL142 and MBNL143 gene isoforms, overexpressed in DM1-patient muscle, encode for nuclear proteins interacting with Src family kinases.

Cell death & disease·2013
Same author

Efficacy of ranolazine in a patient with idiopathic dilated cardiomyopathy and electrical storm.

Drug discoveries & therapeutics·2013
Same author

Assessment of individual lung cancer risk by the proteomic analysis of exhaled breath condensate.

Expert opinion on medical diagnostics·2013

Area of Science:

  • Polymer Chemistry
  • Analytical Chemistry
  • Biophysical Chemistry

Background:

  • Polyacrylamide matrices are widely used in electrophoresis.
  • Understanding ion-matrix interactions is crucial for optimizing separation techniques.
  • Previous studies have not fully elucidated the impact of matrix properties on salt front migration.

Purpose of the Study:

  • To indirectly evaluate the interaction between polyacrylamide matrices and free ions.
  • To investigate the influence of pH and matrix properties on salt front width during electrophoresis.
  • To determine the role of buffering capacity and ionic strength on electrophoretic salt front behavior.

Main Methods:

  • Electrophoresis under standard conditions.
  • Indirect evaluation of ion-matrix interactions via residual salt front width measurement.

Related Experiment Videos

  • Systematic variation of pH, buffering power, and ionic strength of polyacrylamide matrices.

    • Main Results:

    • Salt front width is significantly larger around neutral pH compared to alkaline or acidic conditions.
    • Anions exhibit considerably wider salt fronts than cations across all tested pH values.
    • Matrix buffering power has a greater effect on decreasing front width than ionic strength.
    • Salt front migration slows considerably after 5000 V x h and becomes negligible when diffusion is prevented.
    • Inadequately buffered matrices lead to increased conductivity and reduced electrical field strength due to H+ and OH- comigration.

    Conclusions:

    • The pH and buffering capacity of polyacrylamide matrices are critical factors influencing salt front dynamics in electrophoresis.
    • Anion migration is more sensitive to matrix interactions than cation migration.
    • Optimizing matrix properties can enhance the resolution and efficiency of electrophoretic separations.