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Viral persistence in colorectal cancer cells infected by Newcastle disease virus

Suet-Lin Chia, Khatijah Yusoff, Norazizah Shafee1

  • 1Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia. nshafee@upm.edu.my.

Virology Journal
|June 3, 2014
PubMed
Abstract

Insights

Newcastle disease virus (NDV) can establish persistent infections in cancer cells, leading to treatment resistance. This finding is crucial for optimizing NDV virotherapy strategies in clinical settings.

Area of Science:

  • Oncology
  • Virology
  • Cancer Therapeutics

Background:

  • Newcastle disease virus (NDV) is a promising candidate for cancer virotherapy.
  • Clinical studies have shown promising responses to NDV treatment.
  • The potential for NDV to establish persistent infections in cancer cells remains unexplored.

Purpose of the Study:

  • To investigate the development of persistent Newcastle disease virus (NDV) infection in cancer cells.
  • To determine if persistent NDV infection impacts cancer cell susceptibility to viral killing.

Main Methods:

  • Infection of colorectal cancer cell lines with NDV.
  • Observation of adventitious persistent infection in SW480 cells.
  • Re-infection experiments to assess viral resistance in persistently infected cells.

Main Results:

  • A persistent NDV infection was observed in SW480 cells, designated SW480P.
  • SW480P cells demonstrated resistance to NDV-induced cell killing.
  • Persistently infected SW480P cells retained the NDV genome and produced progeny with reduced plaque-forming ability.

Conclusions:

  • Newcastle disease virus (NDV) can establish persistent infections in cancer cells.
  • Persistent NDV infection can lead to cancer cell resistance to virotherapy.
  • Consideration of persistent infection is essential for effective clinical application of NDV.

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