Quantitative analysis of chlortetracycline and related substances by high-performance liquid chromatography
N H Khan1, E Roets, J Hoogmartens
1Katholieke Universiteit Leuven, Instituut voor Farmaceutische Wetenschappen, Belgium.
Abstract:
Isocratic high-performance liquid chromatography on Zorbax C8 7 microns allows quantitative determination of chlortetracycline, 4-epichlortetracycline, tetracycline, demethylchlortetracycline and isochlortetracycline using a mobile phase containing dimethylsulphoxide, 1 M perchloric acid and water (35:5:60). The minor impurities anhydrochlortetracycline and 4-epianhydrochlortetracycline, which are more strongly retained can be determined using a second isocratic system with a mobile phase containing more organic modifier. The method has been used for the comparison of official standards and for the analysis of a number of commercial samples.
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