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Related Experiment Videos

Rabies-like inclusions in dogs.

J C Nietfeld1, P M Rakich, D E Tyler

  • 1Diagnostic Assistance Laboratory, College of Veterinary Medicine, University of Georgia, Athens 30602.

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
|October 1, 1989
PubMed
Summary

Inclusion bodies resembling Negri bodies were found in nonrabid dogs. Diagnostic tests confirmed these were not rabies virus, highlighting the need for accurate rabies testing methods.

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Area of Science:

  • Veterinary Pathology
  • Virology
  • Canine Infectious Diseases

Background:

  • Inclusion bodies, morphologically similar to Negri bodies, were observed in the brains of eight nonrabid dogs.
  • These findings prompted an investigation to differentiate these inclusions from true Negri bodies associated with rabies.

Purpose of the Study:

  • To investigate the nature of inclusion bodies found in nonrabid dogs.
  • To evaluate the diagnostic accuracy of various methods for rabies detection in the presence of these atypical inclusions.

Main Methods:

  • Immunoperoxidase staining (7 cases), fluorescent antibody (FA) staining (1 case), and transmission electron microscopy (4 cases) were employed.
  • Inclusion bodies were compared with those from confirmed rabies-positive animals.

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Main Results:

  • While some tissues from FA-positive animals showed positive immunoperoxidase staining, all tested brain tissues (immunoperoxidase and FA) from the nonrabid dogs were negative for rabies.
  • Transmission electron microscopy did not detect rabies virus in the inclusion-containing neurons.

Conclusions:

  • The study underscores the critical importance of established diagnostic methods like FA testing and mouse inoculation for accurate rabies diagnosis.
  • Morphologically similar inclusions in nonrabid animals do not indicate rabies infection and can lead to misdiagnosis if not properly evaluated.