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Improvement in in vitro fertilization rate, decrease in reactive oxygen species and spermatozoa death incidence in
A Matini Behzad1, B Ebrahimi2, A R Alizadeh1
1Department of Animal Science, Saveh Branch, Islamic Azad University, Saveh, Iran.
Reproduction in Domestic Animals = Zuchthygiene
|June 4, 2014
Summary
Supplementing ram diets with fish oil improved sperm quality, reduced oxidative stress, and decreased sperm death. This enhances in vitro fertilization rates, offering a nutritional strategy for improving ram fertility.
Area of Science:
- Animal Science
- Reproductive Biology
- Nutritional Science
Background:
- Sperm quality is crucial for successful artificial insemination and natural breeding in livestock.
- Oxidative stress negatively impacts sperm parameters and fertility.
- Dietary interventions, such as fish oil supplementation, are explored to enhance male reproductive health.
Purpose of the Study:
- To investigate the effects of fish oil (FO) supplementation on ram sperm parameters, reactive oxygen species (ROS) levels, and in vitro fertilization (IVF) rates.
- To assess the impact of FO on spermatozoa death incidence and oxidative stress markers.
Main Methods:
- Nine rams were divided into control (CTR) and fish oil (FO) groups, fed for 70 days.
- Semen was collected weekly, and sperm parameters were analyzed using CASA.
- ROS levels and spermatozoa death were assessed via flow cytometry.
- IVF rates were determined by evaluating two-cell stage embryos.
Main Results:
- Fish oil supplementation significantly improved sperm volume, concentration, and progressive motility.
- The FO group exhibited a higher IVF fertilization rate (56%) compared to the CTR group (49%).
- FO reduced superoxide anion (O2(-)) and apoptosis incidence, indicating decreased oxidative stress and cell death.
Conclusions:
- Dietary fish oil supplementation enhances ram sperm quality and reproductive outcomes.
- Fish oil can mitigate oxidative stress and reduce spermatozoa death, contributing to improved fertility.
- This nutritional approach offers a viable strategy for improving ram reproductive efficiency.
