Manual hematoxylin and eosin staining of mouse tissue sections
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Published on: December 12, 2016
Robert D Cardiff1, Claramae H Miller1, Robert J Munn1
1Center for Comparative Medicine and Center for Genomic Pathology, University of California, Davis, Davis, California 95616.
Abstract:
The hematoxylin and eosin (H&E) stain is the standard used for microscopic examination of tissues that have been fixed, processed, embedded, and sectioned. It can be performed manually or by automation. For economic reasons, the manual technique is generally the method of choice for facilities with a low sample volume. This protocol describes manual H&E staining of fixed, processed, paraffin-embedded, and sectioned mouse tissues. In H&E-stained tissues, the nucleic acids stain dark blue and the proteins stain red to pink or orange. For accurate phenotyping and delineation of tissue detail, the protocol must be adhered to rigorously. This includes frequent reagent changes as well as the use of "in-date" reagents. Appropriate color in a good H&E stain allows for identification of many tissue subtleties that are necessary for accurate diagnosis.
Insights
This protocol details manual hematoxylin and eosin (H&E) staining for mouse tissues. Rigorous adherence ensures accurate tissue detail and diagnosis through proper nucleic acid and protein staining.
Area of Science:
- Histology
- Biopathology
- Laboratory Protocols
Background:
- Hematoxylin and eosin (H&E) staining is the gold standard for microscopic tissue examination.
- Manual H&E staining is cost-effective for low-volume facilities.
- Accurate tissue staining is crucial for diagnosis and phenotyping.
Purpose of the Study:
- To describe a protocol for manual H&E staining of fixed, processed, paraffin-embedded, and sectioned mouse tissues.
- To emphasize the importance of rigorous protocol adherence for optimal results.
Main Methods:
- The protocol involves manual H&E staining.
- Key steps include fixation, processing, embedding, and sectioning of mouse tissues.
- Reagent quality and frequent changes are critical.
Main Results:
- H&E staining differentiates nucleic acids (dark blue) and proteins (red/pink/orange).
- Proper staining reveals tissue subtleties essential for accurate diagnosis.
- Adherence to the protocol ensures reliable staining outcomes.
Conclusions:
- Manual H&E staining is a vital technique for histological analysis.
- Strict adherence to the described protocol is necessary for reproducible and accurate results.
- Quality staining facilitates precise tissue interpretation and diagnosis.
